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Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by <t>ELISA.</t> Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.
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Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by <t>ELISA.</t> Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.
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Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by <t>ELISA.</t> Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.
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Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by <t>ELISA.</t> Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.
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Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by ELISA. Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Vitamin D Binding Protein (DBP), Free Calcidiol, and Total Calcitriol in Adults from Northern Greece

doi: 10.61186/rbmb.12.4.652

Figure Lengend Snippet: Mean concentrations of free calcidiol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Free calcidiol was measured by ELISA. Males had significantly greater mean concentration than females in whole sample (p < 0.001) and osteoporosis (p = 0.030), with mild correlations. In total, osteoporosis had a significantly lower mean concentration than type 1 & 2 diabetes, and athletic healthy (p <0.001 in all cases), with very strong correlations. Type 1 & 2 diabetes had a significantly lower mean concentration than athletic healthy (p = 0.017, p = 0.011), with very strong correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, 2: Type 2 diabetes, A: Athletic healthy.

Article Snippet: At the Clinical Chemistry Laboratory, Faculty of Biomedical Sciences, IHU, we measured free calcidiol, total calcitriol, and DBP concentrations, using commercial immunoenzymatic kits: Free 25OH Vitamin D ELISA (Immuno‐Biological Laboratories Inc., USA) for free calcidiol, 1,25(OH)2 Vitamin D ELISA (DIAsource ImmunoAssays, Belgium) for total calcitriol, and Human Vitamin DBP Immunoassay Quantikine® ELISA (China R&D Systems Ltd., China) for DBP, according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Mean concentrations of total calcitriol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Total calcitriol was measured by ELISA. In type 2 diabetes, females had a significantly greater mean concentration than males (p < 0.001) with a strong correlation. In total, osteoporotics had a significantly lower concentration than type 1 diabetics (p = 0.030) and athletic healthy (p = 0.037) with weak correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, A: Athletic healthy.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Vitamin D Binding Protein (DBP), Free Calcidiol, and Total Calcitriol in Adults from Northern Greece

doi: 10.61186/rbmb.12.4.652

Figure Lengend Snippet: Mean concentrations of total calcitriol in the whole sample and the study groups in late winter, in total and by gender. Whole sample: 326 individuals, 161 males and 165 females. Osteoporosis: 99 individuals, 24 males and 75 females. Type 1 diabetes: 53 individuals, 27 males and 26 females. Type 2 diabetes: 51 individuals, 40 males and 11 females. Athletic healthy: 123 individuals, 70 males and 53 females. Total calcitriol was measured by ELISA. In type 2 diabetes, females had a significantly greater mean concentration than males (p < 0.001) with a strong correlation. In total, osteoporotics had a significantly lower concentration than type 1 diabetics (p = 0.030) and athletic healthy (p = 0.037) with weak correlations. *: A statistically significant difference. O: Osteoporosis, 1: Type 1 diabetes, A: Athletic healthy.

Article Snippet: At the Clinical Chemistry Laboratory, Faculty of Biomedical Sciences, IHU, we measured free calcidiol, total calcitriol, and DBP concentrations, using commercial immunoenzymatic kits: Free 25OH Vitamin D ELISA (Immuno‐Biological Laboratories Inc., USA) for free calcidiol, 1,25(OH)2 Vitamin D ELISA (DIAsource ImmunoAssays, Belgium) for total calcitriol, and Human Vitamin DBP Immunoassay Quantikine® ELISA (China R&D Systems Ltd., China) for DBP, according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay

Mean free calcidiol concentrations and the correlations among age subgroups in the whole sample and study groups. Whole sample: Young = 76, middle-aged = 156, elderly = 94. Osteoporosis: Young = 7, middle-aged = 46, elderly = 46. Type 1 diabetes: Young = 34, middle-aged = 18, elderly = 1 (mean concentration not applicable). Type 2 diabetes: Young = 0, middle-aged = 28, elderly = 23. Athletic healthy: Young = 35, middle-aged = 64, elderly = 24. Free calcidiol was measured by ELISA. In the whole sample, the young had a significantly greater mean concentration than the middle-aged (p = 0.004) and the elderly (p < 0,001) with mild correlations. In type 1 diabetes, the young had a significantly greater mean concentration than the middle-aged (p = 0.004) with a strong correlation *: A statistically significant difference.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Vitamin D Binding Protein (DBP), Free Calcidiol, and Total Calcitriol in Adults from Northern Greece

doi: 10.61186/rbmb.12.4.652

Figure Lengend Snippet: Mean free calcidiol concentrations and the correlations among age subgroups in the whole sample and study groups. Whole sample: Young = 76, middle-aged = 156, elderly = 94. Osteoporosis: Young = 7, middle-aged = 46, elderly = 46. Type 1 diabetes: Young = 34, middle-aged = 18, elderly = 1 (mean concentration not applicable). Type 2 diabetes: Young = 0, middle-aged = 28, elderly = 23. Athletic healthy: Young = 35, middle-aged = 64, elderly = 24. Free calcidiol was measured by ELISA. In the whole sample, the young had a significantly greater mean concentration than the middle-aged (p = 0.004) and the elderly (p < 0,001) with mild correlations. In type 1 diabetes, the young had a significantly greater mean concentration than the middle-aged (p = 0.004) with a strong correlation *: A statistically significant difference.

Article Snippet: At the Clinical Chemistry Laboratory, Faculty of Biomedical Sciences, IHU, we measured free calcidiol, total calcitriol, and DBP concentrations, using commercial immunoenzymatic kits: Free 25OH Vitamin D ELISA (Immuno‐Biological Laboratories Inc., USA) for free calcidiol, 1,25(OH)2 Vitamin D ELISA (DIAsource ImmunoAssays, Belgium) for total calcitriol, and Human Vitamin DBP Immunoassay Quantikine® ELISA (China R&D Systems Ltd., China) for DBP, according to the manufacturer’s instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Mean total calcitriol concentrations among age subgroups in the whole sample and study groups. Whole sample: Young = 76, middle-aged = 156, elderly = 94. Osteoporosis: Young = 7, middle-aged = 46, elderly = 46. Type 1 diabetes: Young = 34, middle-aged = 18, elderly = 1 (mean concentration not applicable). Type 2 diabetes: Young = 0, middle-aged = 28, elderly = 23. Athletic healthy: Young = 35, middle-aged = 64, elderly = 24. Total calcitriol was measured by ELISA. In type 2 diabetics, the elderly had a significantly greater mean concentration than the middle-aged (p = 0.006) with a mild to strong correlation. No other correlations were found.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Vitamin D Binding Protein (DBP), Free Calcidiol, and Total Calcitriol in Adults from Northern Greece

doi: 10.61186/rbmb.12.4.652

Figure Lengend Snippet: Mean total calcitriol concentrations among age subgroups in the whole sample and study groups. Whole sample: Young = 76, middle-aged = 156, elderly = 94. Osteoporosis: Young = 7, middle-aged = 46, elderly = 46. Type 1 diabetes: Young = 34, middle-aged = 18, elderly = 1 (mean concentration not applicable). Type 2 diabetes: Young = 0, middle-aged = 28, elderly = 23. Athletic healthy: Young = 35, middle-aged = 64, elderly = 24. Total calcitriol was measured by ELISA. In type 2 diabetics, the elderly had a significantly greater mean concentration than the middle-aged (p = 0.006) with a mild to strong correlation. No other correlations were found.

Article Snippet: At the Clinical Chemistry Laboratory, Faculty of Biomedical Sciences, IHU, we measured free calcidiol, total calcitriol, and DBP concentrations, using commercial immunoenzymatic kits: Free 25OH Vitamin D ELISA (Immuno‐Biological Laboratories Inc., USA) for free calcidiol, 1,25(OH)2 Vitamin D ELISA (DIAsource ImmunoAssays, Belgium) for total calcitriol, and Human Vitamin DBP Immunoassay Quantikine® ELISA (China R&D Systems Ltd., China) for DBP, according to the manufacturer’s instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

Free calcidiol and total calcitriol mean concentrations and their correlations in late winter and late summer among the whole of subjects with a second measurement and the study groups. Whole sample: 106 individuals. Osteoporosis: 35 individuals. Diabetes: 20 individuals. Athletic healthy: 51 individuals. Free calcidiol and total calcitriol were measured by ELISA. In osteoporotics, free calcidiol concentration was significantly greater in the winter (p < 0.001) with a strong correlation, whereas total calcitriol concentration was significantly greater in the summer (p = 0.049) with a weak correlation. In diabetics, both free calcidiol and total calcitriol was significantly greater in summer (p = 0.005 with a mild correlation, and p < 0.001 with a strong correlation). In athletic healthy subjects, free calcidiol was significantly greater in summer (p < 0.001) with a strong correlation, whereas total calcitriol was significantly lower in summer (p < 0.001) with a strong correlation. *: A statistically significant difference. W: Winder. S: Summer.

Journal: Reports of Biochemistry & Molecular Biology

Article Title: Vitamin D Binding Protein (DBP), Free Calcidiol, and Total Calcitriol in Adults from Northern Greece

doi: 10.61186/rbmb.12.4.652

Figure Lengend Snippet: Free calcidiol and total calcitriol mean concentrations and their correlations in late winter and late summer among the whole of subjects with a second measurement and the study groups. Whole sample: 106 individuals. Osteoporosis: 35 individuals. Diabetes: 20 individuals. Athletic healthy: 51 individuals. Free calcidiol and total calcitriol were measured by ELISA. In osteoporotics, free calcidiol concentration was significantly greater in the winter (p < 0.001) with a strong correlation, whereas total calcitriol concentration was significantly greater in the summer (p = 0.049) with a weak correlation. In diabetics, both free calcidiol and total calcitriol was significantly greater in summer (p = 0.005 with a mild correlation, and p < 0.001 with a strong correlation). In athletic healthy subjects, free calcidiol was significantly greater in summer (p < 0.001) with a strong correlation, whereas total calcitriol was significantly lower in summer (p < 0.001) with a strong correlation. *: A statistically significant difference. W: Winder. S: Summer.

Article Snippet: At the Clinical Chemistry Laboratory, Faculty of Biomedical Sciences, IHU, we measured free calcidiol, total calcitriol, and DBP concentrations, using commercial immunoenzymatic kits: Free 25OH Vitamin D ELISA (Immuno‐Biological Laboratories Inc., USA) for free calcidiol, 1,25(OH)2 Vitamin D ELISA (DIAsource ImmunoAssays, Belgium) for total calcitriol, and Human Vitamin DBP Immunoassay Quantikine® ELISA (China R&D Systems Ltd., China) for DBP, according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay